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cd44  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cd44
    Cd44, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44+antibody/CD44+Antibody/pmc13052523-51-17-26
    Average 96 stars, based on 789 article reviews
    cd44 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: A conditionally replicative adenovirus vector containing the synNotch receptor gene for the treatment of muscle-invasive bladder cancer
    Article Snippet: Immunohistochemical staining was performed in an automatic tissue processor (Leica Microsystems Bond). .. Briefly, tissue sections were incubated at RT for 15 min with an anti-CD44 antibody (1:600, Catalog#: 37259S, Cell Signaling Technology). ..

    Article Title: A conditionally replicative adenovirus vector containing the synNotch receptor gene for the treatment of muscle-invasive bladder cancer.
    Article Snippet: Immunohistochemical staining was performed in an automatic tissue processor (Leica Microsystems Bond). .. Briefly, tissue sections were incubated at RT for 15min with an anti-CD44 antibody (1:600, Catalog#: 37259S, Cell Signaling Technology). ..

    Article Title: Silencing ZIC5 suppresses glycolysis and promotes disulfidptosis in lung adenocarcinoma cells
    Article Snippet: The data were analyzed using FlowJo software (FlowJo LLC, version 10.8.1). .. Cells were fixed and permeabilized with 0.1% Triton X-100 (Sigma, T8787), then blocked with 5% BSA (Sigma, A2153) for 1 h. The cells were incubated overnight with anti-CD44 antibody (Cell Signaling Technology, 3570S, 1:200) and anti-actin antibody (Sigma, A2066, 1:200). .. The next day, cells were incubated at room temperature for 1 h with Alexa Fluor 488-conjugated secondary antibody (Invitrogen, A-11008, 1:500) and Alexa Fluor 594-conjugated secondary antibody (Invitrogen, A-11012, 1:500).

    Article Title: Silencing ZIC5 suppresses glycolysis and promotes disulfidptosis in lung adenocarcinoma cells.
    Article Snippet: The data were analyzed using FlowJo software (FlowJo LLC, version 10.8.1). .. Immunofluorescence staining for CD44 and actin expression Cells were fixed and permeabilized with 0.1% Triton X-100 (Sigma, T8787), then blocked with 5% BSA (Sigma, A2153) for 1 h. The cells were incubated overnight with anti-CD44 antibody (Cell Signaling Technology, 3570S, 1:200) and anti-actin antibody (Sigma, A2066, 1:200). .. The next day, cells were incubated at room temperature for 1 h with Alexa Fluor 488- conjugated secondary antibody (Invitrogen, A-11008, 1:500) and Alexa Fluor 594-conjugated secondary antibody (Invitrogen, A-11012, 1:500).

    other:

    Article Title: Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancer
    Article Snippet: Anti-CD44 antibody , Cell Signaling , Cat# 37259; RRID: AB_2750879.

    Immunofluorescence:

    Article Title: Hypertension and age‐related focal global glomerulosclerosis are associated with biomarkers for cellular senescence
    Article Snippet: .. Cytokeratin 7 (CK7) and CD44 immunofluorescence were performed using mouse monoclonal anti‐CK7 antibody (1:10 dilution; GA61961‐2, Agilent, Santa Clara, CA) and mouse monoclonal anti‐CD44 antibody (1:200 dilution, 5640S, Cell Signaling Technology, Danvers, MA), respectively. .. Each procedure included co‐staining with anti‐annexin A3 (anti‐ANXA3) (1:200 dilution, HPA013398, Sigma‐Aldrich, St Louis, MO) as a PEC marker and DAPI (1:200 dilution, D9542, Sigma‐Aldrich) for nuclei.

    Article Title: Silencing ZIC5 suppresses glycolysis and promotes disulfidptosis in lung adenocarcinoma cells.
    Article Snippet: The data were analyzed using FlowJo software (FlowJo LLC, version 10.8.1). .. Immunofluorescence staining for CD44 and actin expression Cells were fixed and permeabilized with 0.1% Triton X-100 (Sigma, T8787), then blocked with 5% BSA (Sigma, A2153) for 1 h. The cells were incubated overnight with anti-CD44 antibody (Cell Signaling Technology, 3570S, 1:200) and anti-actin antibody (Sigma, A2066, 1:200). .. The next day, cells were incubated at room temperature for 1 h with Alexa Fluor 488- conjugated secondary antibody (Invitrogen, A-11008, 1:500) and Alexa Fluor 594-conjugated secondary antibody (Invitrogen, A-11012, 1:500).

    Concentration Assay:

    Article Title:
    Article Snippet: .. Anti-CD44 antibody clone information Antibodies Source Catalog # Clone Isotype Concentration CD44 Cell Signaling Technology (CST) 3570 156-3C11 mIgG2a 0.215 ug/ml CD44 Abcam ab51037 EPR1013Y rIgG 0.192 ug/ml Cytokeratin DAKO M351501-2 AE1/AE3 mIgG1 0.157 ug/ml Cytokeratin DAKO Z062201-2 Polyclonal rIgG 123 ug/ml doi: 10.1136/jitc-2022-004757:e004757. ..

    Staining:

    Article Title: Silencing ZIC5 suppresses glycolysis and promotes disulfidptosis in lung adenocarcinoma cells.
    Article Snippet: The data were analyzed using FlowJo software (FlowJo LLC, version 10.8.1). .. Immunofluorescence staining for CD44 and actin expression Cells were fixed and permeabilized with 0.1% Triton X-100 (Sigma, T8787), then blocked with 5% BSA (Sigma, A2153) for 1 h. The cells were incubated overnight with anti-CD44 antibody (Cell Signaling Technology, 3570S, 1:200) and anti-actin antibody (Sigma, A2066, 1:200). .. The next day, cells were incubated at room temperature for 1 h with Alexa Fluor 488- conjugated secondary antibody (Invitrogen, A-11008, 1:500) and Alexa Fluor 594-conjugated secondary antibody (Invitrogen, A-11012, 1:500).

    Expressing:

    Article Title: Silencing ZIC5 suppresses glycolysis and promotes disulfidptosis in lung adenocarcinoma cells.
    Article Snippet: The data were analyzed using FlowJo software (FlowJo LLC, version 10.8.1). .. Immunofluorescence staining for CD44 and actin expression Cells were fixed and permeabilized with 0.1% Triton X-100 (Sigma, T8787), then blocked with 5% BSA (Sigma, A2153) for 1 h. The cells were incubated overnight with anti-CD44 antibody (Cell Signaling Technology, 3570S, 1:200) and anti-actin antibody (Sigma, A2066, 1:200). .. The next day, cells were incubated at room temperature for 1 h with Alexa Fluor 488- conjugated secondary antibody (Invitrogen, A-11008, 1:500) and Alexa Fluor 594-conjugated secondary antibody (Invitrogen, A-11012, 1:500).



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    Miltenyi Biotec cd44 antibody
    Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and <t>CD44</t> cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.
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    Cell Signaling Technology Inc cd44
    Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and <t>CD44</t> cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.
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    Image Search Results


    Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

    Journal: STAR Protocols

    Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

    doi: 10.1016/j.xpro.2026.104519

    Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

    Article Snippet: CD44 Antibody, anti-mouse, PE-Vio 770 clone IM7.8.1; 1:10 dilution , Miltenyi Biotec , Cat# 130-102-377 RRID: AB_2658187.

    Techniques: Cell Culture, Dot Blot, Luminex